Protirelin (TRH Thyrotropin)

Description

Overview of Thyrotropin Protirelin

Protirelin, also referred to as thyrotropin–releasing hormone (TRH), is a naturally occurring tripeptide neuropeptide composed of L–pyroglutamyl–L–histidyl–L–prolinamide. It was first identified as a hypothalamic neurohormone that initiates regulatory signals within the hypothalamic–pituitary axis. In biochemical research, protirelin is widely used as a reference peptide in experimental models of receptor signaling and neuroendocrine regulation because of its well‑defined molecular identity and receptor interactions

Chemical and Molecular Properties 

Thyrotropin Protirelin
PubChem CID	638678
Molecular Formula	C16H22N6O4
Molecular Weight	362.38 g/mol
Synonyms	protirelin 24305-27-9 Thyroliberin Lopremone Synthetic TRH
IUPAC	(2S)-N-[(2S)-1-[(2S)-2-carbamoylpyrrolidin-1-yl]-3-(1H-imidazol-5-yl)-1-oxopropan-2-yl]-5-oxopyrrolidine-2-carboxamide
CAS	24305-27-9
Labeling	Research Use Only (RUO), not for human or animal consumption.
Chemical Structure Depiction
Purity	≥98%
Classification	Research Use Only (RUO)
Storage Temperature	Store at −18 °C or lower
Solubility	Soluble in water and aqueous research solvents
Safety	Handle with gloves, a lab coat, eye protection; use a fume hood if dust/aerosol is possible

Various Mechanisms of Protirelin

TRH Receptor Interaction

Protirelin binds to thyrotropin–releasing hormone receptors (TRHRs), which are members of the class A G protein‑coupled receptor (GPCR) family. TRHR engagement has been characterized in cell‑based receptor pharmacology models and structural studies. Upon ligand binding, TRHR undergoes conformational changes that enable coupling to Gq/G₁₁ proteins, initiating downstream signaling pathways.

GPCR Signal Transduction

Activation of TRHR by protirelin leads to stimulation of phospholipase C (PLC) activity. PLC catalyzes the hydrolysis of membrane phosphatidylinositol bisphosphate to produce:

• Inositol–1,4,5–trisphosphate (IP₃)
• Diacylglycerol (DAG)

These second messengers are hallmarks of GPCR–mediated signal transduction in biochemical studies. IP₃ induces the release of intracellular Ca²⁺ ions, while DAG participates in the activation of protein kinase C (PKC) pathways.

Intracellular Calcium Mobilization

IP₃–mediated calcium release from internal stores results in elevated intracellular calcium concentrations in experimental models. Increased Ca²⁺ is a key signal in cellular response cascades and is routinely measured in receptor activation assays to characterize protirelin‑dependent signaling. 

Research Applications of Protirelin

Protirelin is utilized as an investigational research peptide in laboratory and preclinical studies. It serves primarily to examine TRH receptor signaling, GPCR activation, and calcium–dependent intracellular pathways. Researchers also employ it to explore neuroendocrine regulation, peptide–receptor interactions, and neurotransmitter-related signaling in in vitro systems and experimental models.

Why Choose Purerawz for Protirelin?

Buy Protirelin for laboratory research use from our online shop. At Purerawz, we provide high-quality reference materials. Each research compound comes with a Certificate of Analysis for verification of purity and concentration.

Note:

Protirelin is an investigational compound currently undergoing clinical evaluation and has not been established as safe or effective for any therapeutic use

Disclaimer

This information is for educational purposes only and not medical advice. Products are for research use only. Research must follow IRB or IACUC guidelines. Verify information independently before purchasing. By ordering, you agree to our Terms and Conditions. If you are not 100% satisfied with the product you received, please contact us at support@purerawz.co

ATTENTION: All our products are for LABORATORY AND RESEARCH PURPOSES ONLY, not for veterinary or human use

Reference Links

Chiba T., Yamada M., Hashimoto Y., et al. Development of a femtomolar‑acting humanin derivative named Colivelin by attaching activity–dependent neurotrophic factor to its N terminus: characterization of Colivelin–mediated neuroprotection against Alzheimer’s disease–relevant insults in vitro and in vivo. Journal of Neuroscience. 25(44):10252–10261. https://pubmed.ncbi.nlm.nih.gov/16267233/

Arakawa T., Niikura T., Arisaka F., et al. Activity‑dependent neurotrophic factor, ADNF, determines the structure characteristics of Colivelin, a fusion protein of ADNF9 and Humanin analog. Journal of Peptide Science. 14(5):631–636. https://pubmed.ncbi.nlm.nih.gov/17994638/

Matsuoka M., Hashimoto Y., Aiso S., et al. Humanin and Colivelin: neuronal–death–suppressing peptides for Alzheimer’s disease and amyotrophic lateral sclerosis. CNS Drug Reviews. 12(2):113–122. https://pubmed.ncbi.nlm.nih.gov/16958985/

PubChem Compound Summary for CID 638678. PubChem. National Center for Biotechnology Information, U.S. National Library of Medicine. https://pubchem.ncbi.nlm.nih.gov/compound/638678#section=Molecular-Formula